Mouse CXCL17 ELISA Kit PicoKine®, Carrier-free

About this product

Mouse CXCL17 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cxcl17 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1768-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Chemokine (C-X-C motif) ligand 17 (CXCL17) is a small cytokine belonging to the CXC chemokine family that has been identified in humans and mice. It is mapped to 7; 7 A3. The protein encoded by this gene is a mucosal chemokine that attracts immature dendritic cells and blood monocytes to the lungs. The encoded protein also promotes tumorigenesis through an angiogenic activity. Finally, this protein exhibits strong antimicrobial activity against E. coli, S. aureus, Salmonella, P. aeruginosa, and C. albicans. Two transcript variants, one protein-coding and the other non-protein coding, have been found for this gene.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>254</td><td>163</td><td>309</td><td>180</td><td>227</td><td>7.59</td><td>3.4%</td></tr><tr><td>Sample 2</td><td>313</td><td>396</td><td>389</td><td>313</td><td>353</td><td>24.27</td><td>6.9%</td></tr><tr><td>Sample 3</td><td>3010</td><td>4228</td><td>2934</td><td>3528</td><td>3425</td><td>160.29</td><td>4.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

C-X-C motif chemokine 17; 6-Cys CXCL17; 13.6 kDa protein; VEGF coregulated chemokine 1; 4-Cys CXCL17; Cxcl17; Vcc1

Gene Name

chemokine (C-X-C motif) ligand 17

Gene ID

232983

UniProt

Q5UW37

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S23-L119

Tissue Specificity

Detected in lung, trachea, lung, tongue thyroid, submaxillary gland, epididymis, and uterus tissues and at a lower level in ovary, prostate and in intestinal tissues.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

Chemokine that acts as chemoattractant for monocytes, macrophages and dendritic cells (PubMed:24973458). Plays a role in angiogenesis and possibly in the development of tumors (PubMed:16989774). Acts as an anti-inflammatory in the stomach. May play a role in the innate defense against infections. Activates the C-X-C chemokine receptor GPR35 to induce a rapid and transient rise in the level of intracellular calcium ions.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse CXCL17

Anticoagulant

heparin, EDTA or citrate

Subcellular Location

Secreted.