Human SPOCK1/Testican-1 ELISA Kit PicoKine®, Carrier-free

About this product

Human SPOCK1/Testican-1 ELISA Kit PicoKine® (96 Tests). Quantitate Human SPOCK1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1848-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Testican-1 is a protein that in humans is encoded by the SPOCK1 gene. It is mapped to 5q31.2. This gene encodes the protein core of a seminal plasma proteoglycan containing chondroitin- and heparan-sulfate chains. The protein's function is unknown, although similarity to thyropin-type cysteine protease-inhibitors suggests its function may be related to protease inhibition.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>21</td><td>23</td><td>22</td><td>20</td><td>21</td><td>1.11</td><td>5.2%</td></tr><tr><td>Sample 2</td><td>67</td><td>71</td><td>74</td><td>66</td><td>69</td><td>3.20</td><td>4.6%</td></tr><tr><td>Sample 3</td><td>435</td><td>409</td><td>375</td><td>396</td><td>403</td><td>21.74</td><td>5.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Testican-1; Protein SPOCK; SPOCK1; SPOCK; TIC1; TICN1

Gene Name

SPARC (osteonectin), cwcv and kazal like domains proteoglycan 1

Gene ID

6695

UniProt

Q08629

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: R22-W439

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<10 pg/ml

Function

May play a role in cell-cell and cell-matrix interactions. May contribute to various neuronal mechanisms in the central nervous system.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human SPOCK1/Testican-1

Anticoagulant

heparin or EDTA

Subcellular Location

Extracellular matrix.