Human Renalase ELISA Kit PicoKine®, Carrier-free

About this product

Human Renalase ELISA Kit PicoKine® (96 Tests). Quantitate Human RNLS in cell culture supernatants, serum and plasma (heparin). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1951-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Renalase, FAD-dependent amine oxidase is an enzyme that in humans is encoded by the RNLS gene. It is mapped to 10q23.31. Renalase is a flavin adenine dinucleotide-dependent amine oxidase that is secreted into the blood from the kidney. The renalase protein consists of a putative secretory signal peptide (SignalP score of 0.4), a flavin adenine dinucleotide (FAD)-binding region, and an oxidase domain. At least four alternative splicing isoforms have been identified in humans (hRenalase1 to hRenalase4). Only hRenalase1 is detected in human blood samples, which means that hRenalase2 to 4 probably have different functions than hRenalase1.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>181</td><td>171</td><td>175</td><td>177</td><td>176</td><td>3.60</td><td>2.0%</td></tr><tr><td>Sample 2</td><td>1100</td><td>1074</td><td>1106</td><td>1218</td><td>1124</td><td>55.30</td><td>4.9%</td></tr><tr><td>Sample 3</td><td>3799</td><td>3638</td><td>3667</td><td>3825</td><td>3732</td><td>80.93</td><td>2.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

Renalase; Monoamine oxidase-C; MAO-C; RNLS; C10orf59

Gene Name

renalase, FAD dependent amine oxidase

Gene ID

55328

UniProt

Q5VYX0

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A18-I342

Tissue Specificity

Secreted into the blood by the kidney. Highly expressed in the kidney, expressed at lower level in heart, skeletal muscle and small intestine. Its plasma concentration is markedly reduced in patients with end-stage renal disease, as compared with healthy subjects.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

Catalyzes the oxidation of the less abundant 1,2-dihydro-beta-NAD (P) and 1,6-dihydro-beta-NAD (P) to form beta-NAD (P)+. The enzyme hormone is secreted by the kidney, and circulates in blood and modulates cardiac function and systemic blood pressure. Lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human Renalase

Anticoagulant

heparin

Subcellular Location

Secreted.