Mouse/Rat DCC ELISA Kit PicoKine®, Carrier-free

About this product

Mouse/Rat DCC ELISA Kit PicoKine® (96 Tests). Quantitate Mouse/Rat Dcc in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2184-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Netrin receptor DCC, also known as DCC, or colorectal cancer suppressor is a protein which in humans is encoded by the DCC gene. Predicted to enable identical protein binding activity; netrin receptor activity; and transcription coactivator activity. Involved in postsynaptic modulation of chemical synaptic transmission. Acts upstream of or within generation of neurons. Located in axon. Is integral component of postsynaptic density membrane. Is active in Schaffer collateral - CA1 synapse. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; lung; and retina. Human ortholog(s) of this gene implicated in colorectal cancer; congenital mirror movement disorder; esophageal cancer; and esophagus squamous cell carcinoma. Orthologous to human DCC (DCC netrin 1 receptor).

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>13</td><td>15</td><td>18</td><td>22</td><td>17</td><td>1.17</td><td>6.9%</td></tr><tr><td>Sample 2</td><td>98</td><td>102</td><td>113</td><td>97</td><td>103</td><td>6.66</td><td>6.5%</td></tr><tr><td>Sample 3</td><td>265</td><td>278</td><td>296</td><td>282</td><td>280</td><td>18.5</td><td>6.6%</td></tr></table>*number of samples for each test n=16.

Gene Name

Dcc

Gene ID

13176

UniProt

P70211/Q63155

Reactivity

Mouse,Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: F32-N1097

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

23.4 pg/ml - 1,500 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant Mouse/Rat DCC