Human DPP7 ELISA Kit PicoKine®, Carrier-free

About this product

Human DPP7 ELISA Kit PicoKine® (96 Tests). Quantitate Human DPP7 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2186-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Dipeptidyl-peptidase 2 is an enzyme that in humans is encoded by the DPP7 gene. The protein encoded by this gene is a post-proline cleaving aminopeptidase expressed in quiescent lymphocytes. The resting lymphocytes are maintained through suppression of apoptosis, a state which is disrupted by inhibition of this novel serine protease. The enzyme has strong sequence homology with prolylcarboxypeptidase and is active at both acidic and neutral pH.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>86</td><td>81</td><td>85</td><td>89</td><td>85</td><td>2.73</td><td>3.2%</td></tr><tr><td>Sample 2</td><td>212</td><td>226</td><td>208</td><td>215</td><td>215</td><td>9.47</td><td>4.4%</td></tr><tr><td>Sample 3</td><td>922</td><td>965</td><td>942</td><td>951</td><td>945</td><td>38.75</td><td>4.1%</td></tr></table>*number of samples for each test n=16.

Gene Name

DPP7

Gene ID

29952

UniProt

Q9UHL4

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G22-L492

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant Human DPP7