Human Jagged1 ELISA Kit PicoKine®, Carrier-free

About this product

Human Jagged1 PicoKine® ELISA Kit (96 Tests). Quantitate Human Jagged1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 30 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2224-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter is involved in signaling processes. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>90</td><td>96</td><td>75</td><td>67</td><td>82</td><td>4.44</td><td>5.4%</td></tr><tr><td>Sample 2</td><td>185</td><td>143</td><td>239</td><td>169</td><td>184</td><td>12.6</td><td>6.9%</td></tr><tr><td>Sample 3</td><td>1704</td><td>1270</td><td>1116</td><td>1693</td><td>1446</td><td>93.40</td><td>6.5%</td></tr></table>*number of samples for each test n=16.

Gene Name

JAG1

Gene ID

182

UniProt

P78504

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: S32-S1046

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<30 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human JAG1

Anticoagulant

heparin or EDTA