Human Intergrin Alpha 5/CD49E ELISA Kit PicoKine®, Carrier-free

About this product

Human Intergrin Alpha 5/CD49E PicoKine® ELISA Kit (96 Tests). Quantitate Human Intergrin Alpha 5/CD49E in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 75 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2227-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Integrin alpha-5 is a protein that in humans is encoded by the ITGA5 gene. The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>242</td><td>166</td><td>180</td><td>215</td><td>201</td><td>9.23</td><td>4.6%</td></tr><tr><td>Sample 2</td><td>502</td><td>527</td><td>467</td><td>385</td><td>470</td><td>32.92</td><td>7%</td></tr><tr><td>Sample 3</td><td>2865</td><td>4075</td><td>4582</td><td>2563</td><td>3521</td><td>196.49</td><td>5.6%</td></tr></table>*number of samples for each test n=16.

Gene Name

ITGA5

Gene ID

3678

UniProt

P08648

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: F42-Y995

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<75 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human ITGA5

Anticoagulant

heparin or EDTA