Human MEGF9 ELISA Kit PicoKine®, Carrier-free

About this product

Human MEGF9 ELISA Kit PicoKine® (96 Tests). Quantitate Human MEGF9 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 75 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2278-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

MEGF9 (Multiple EGF?like domains protein 9; also EGF?like protein 5) is a 63 kDa (predicted) novel transmembrane glycoprotein that shares some homology to beta?chains of laminin. It is expressed by hepatocytes, cerebellar Purkinje cells, Schwann cells, keratinocytes and intestinal epithelium. MEGF9 is suggested to participate in cell motility, and its absence correlates with tumor cell migration. Mature human MEGF9 is 572 amino acids (aa) in length. It is a single span type I transmembrane protein that contains a 484 aa extracellular region (aa 31?514) plus a 68 aa C?terminal cytoplasmic domain. The extracellular region possesses a lengthy Pro?rich region (aa 55?200), followed by five EGF?like domains (aa 204?451). MEGF9 may run at approximately 160 kDa in SDS?PAGE, suggesting either heavy glycosylation or dimerization. Over aa 36?514, human MEGF9 shares 76% aa sequence identity with mouse MEGF9.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>223</td><td>215</td><td>175</td><td>306</td><td>230</td><td>16.96</td><td>7.4%</td></tr><tr><td>Sample 2</td><td>315</td><td>457</td><td>345</td><td>413</td><td>383</td><td>26.12</td><td>6.8%</td></tr><tr><td>Sample 3</td><td>3772</td><td>4291</td><td>2918</td><td>4826</td><td>3952</td><td>199.17</td><td>5%</td></tr></table>*number of samples for each test n=16.

Gene Name

MEGF9

Gene ID

1955

UniProt

Q9H1U4

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A36-N514

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<75 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human MEGF9

Anticoagulant

heparin or EDTA