Human MEPE ELISA Kit PicoKine®, Carrier-free

About this product

Human MEPE ELISA Kit PicoKine® (96 Tests). Quantitate Human MEPE in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 75 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2279-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Matrix extracellular phosphoglycoprotein (Osteoblast/osteocyte factor 45) is a protein that in humans is encoded by the MEPE gene. This gene encodes a secreted calcium-binding phosphoprotein that belongs to the small integrin-binding ligand, N-linked glycoprotein (SIBLING) family of proteins. Members of this family are components of the extracellular matrix of bone and dentin and regulate bone mineralization. Deficiency of a similar protein in mouse results in increased bone mass. Mice lacking this gene are resistant to aging-related trabecular bone loss. Alternative splicing results in multiple transcript variants.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>224</td><td>226</td><td>307</td><td>234</td><td>248</td><td>18.01</td><td>7.3%</td></tr><tr><td>Sample 2</td><td>462</td><td>335</td><td>340</td><td>457</td><td>399</td><td>19.57</td><td>4.9%</td></tr><tr><td>Sample 3</td><td>4230</td><td>4343</td><td>3648</td><td>4613</td><td>4209</td><td>247.88</td><td>5.9%</td></tr></table>*number of samples for each test n=16.

Gene Name

MEPE

Gene ID

56955

UniProt

Q9NQ76

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: P18-D525

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<75 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human MEPE

Anticoagulant

heparin or EDTA