Human C-Peptide ELISA Kit PicoKine®, Carrier-free

About this product

Human C-Peptide ELISA Kit PicoKine® (96 Tests). Quantitate Human C-Peptide in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2288-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

C-Peptide, or connecting peptide, is a short peptide chain that is produced during the synthesis of insulin. When proinsulin is cleaved to form insulin, C-Peptide is released as a byproduct. It serves as a useful biomarker for assessing insulin production in the body because its levels reflect the amount of insulin secreted by the pancreas. Unlike insulin, C-Peptide has a longer half-life in the bloodstream, making it easier to measure. Elevated levels of C-Peptide can indicate insulinoma or insulin resistance, while low levels may suggest type 1 diabetes or pancreatic dysfunction. Understanding C-Peptide levels can help in diagnosing and managing various metabolic disorders.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>69</td><td>72</td><td>106</td><td>71</td><td>80</td><td>5.23</td><td>6.6%</td></tr><tr><td>Sample 2</td><td>318</td><td>500</td><td>455</td><td>447</td><td>430</td><td>15.01</td><td>3.5%</td></tr><tr><td>Sample 3</td><td>1720</td><td>1706</td><td>1260</td><td>1331</td><td>1504</td><td>117.63</td><td>7.8%</td></tr></table>*number of samples for each test n=16.

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: human peptide

Applications

ELISA

Standard

2 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|5

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Anticoagulant

heparin or EDTA