Human IFN Gamma/IFNG/Interferon Gamma PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human IFN Gamma/IFNG/Interferon Gamma PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate human IFNG in cell culture supernatants and serum. Sensitivity: 2 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0373-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Interferon-gamma(IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer(NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.

Reproducibility

<table class="intra_inter_assay_variability"> <tbody> <tr><th></th><th colspan="3">Intra-Assay Precision</th><th colspan="3">Inter-Assay Precision</th></tr><tr><td>Sample</td><td>1</td><td>2</td><td>3</td><td>1</td><td>2</td><td>3</td></tr><tr><td>n</td><td>16</td><td>16</td><td>16</td><td>24</td><td>24</td><td>24</td></tr><tr><td>Mean (pg/ml)</td><td>34</td><td>150</td><td>469</td><td>33</td><td>139</td><td>437</td></tr><tr><td>Standard deviation</td><td>1.73</td><td>8.7</td><td>29.54</td><td>1.91</td><td>9.73</td><td>31.9</td></tr><tr><td>CV (%)</td><td>7.3%</td><td>5.8%</td><td>7%</td><td>5.8%</td><td>7%</td><td>7.3%</td></tr> </tbody></table>

Gene Name

IFNG

Gene ID

3458

UniProt

P01579

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q24-Q166

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants and serum.

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<2 pg/ml

Components

Description|Quantity|Volume Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 HRP-Linked Antibody|1|6ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)