Human ACE PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human ACE/Cd143 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human ACE in cell culture supernatants, serum, plasma (heparin) and saliva. Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0557-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Angiotensin-converting enzyme (ACE) is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is thought to play a critical role in blood pressure regulation. The predicted protein is identical, from residue 37 to its C terminus, to the second half or C-terminal domain of the endothelial ACE sequence. The protein sequence inferred consists of a 732-residue preprotein including a 31-residue signal peptide. The mature polypeptide has a molecular weight of 80,073. Although ACE has been studied primarily in the context of its role in blood pressure regulation, this widely distributed enzyme has many other physiological functions. The ACE gene encodes two isozymes. The somatic isozyme is expressed in many tissues, including vascular endothelial cells, renal epithelial cells, and testicular Leydig cells, whereas the testicular or germinal angiotensin-converting enzyme is expressed only in sperm. The standard product used in this kit is recombinant human ACE, consisting of 30-1261 amino acids with the molecular mass of 120KDa.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>1278</td><td>1310</td><td>1292</td><td>1425</td><td>1326</td><td>58.13</td><td>4.3%</td></tr><tr><td>Sample 2</td><td>6839</td><td>6994</td><td>7039</td><td>6669</td><td>6885</td><td>145.23</td><td>2.1%</td></tr><tr><td>Sample 3</td><td>24157</td><td>24884</td><td>22207</td><td>21600</td><td>23212</td><td>1350.66</td><td>5.8%</td></tr></table>*number of samples for each test n=16.

Gene Name

angiotensin I converting enzyme

Gene ID

1636

UniProt

P12821

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L30-L1261

Applications

ELISA

Standard

50 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin) and saliva.

Detection Range

0.78 ng/ml - 50 ng/ml

Sensitivity

<5 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

53750 MW

Specificity

Natural and recombinant human ACE

Anticoagulant

heparin

Protein Name

Angiotensin-converting enzyme