Human CA9 PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human CA9/Ca Ix PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human CA9 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0682-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Carbonic anhydrase 9 (CA9/CAIX) is an enzyme that in humans is encoded by the CA9 gene. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene is mapped to 9p13.3. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>37</td><td>32</td><td>32</td><td>33</td><td>33</td><td>2.06</td><td>6.2%</td></tr><tr><td>Sample 2</td><td>196</td><td>230</td><td>215</td><td>208</td><td>212</td><td>12.29</td><td>5.7%</td></tr><tr><td>Sample 3</td><td>412</td><td>423</td><td>456</td><td>402</td><td>423</td><td>20.31</td><td>4.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Carbonic anhydrase 9;4.2.1.1;Carbonate dehydratase IX;Carbonic anhydrase IX;CA-IX;CAIX;Membrane antigen MN;P54/58N;Renal cell carcinoma-associated antigen G250;RCC-associated antigen G250;pMW1;CA9;G250, MN;

Gene Name

Carbonic anhydrase 9

Gene ID

768

UniProt

Q16790

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: P59-D414

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

49698 MW

Specificity

Natural and recombinant human CA9

Anticoagulant

heparin, EDTA or citrate

Protein Name

Carbonic anhydrase 9