Human CD32 PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human Fc gamma RIIB/C (CD32b/c) (FCGR2B/C) PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human FCGR2B/C in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK1312-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>122</td><td>125</td><td>105</td><td>106</td><td>114</td><td>9.06</td><td>7.9%</td></tr><tr><td>Sample 2</td><td>404</td><td>361</td><td>357</td><td>357</td><td>369</td><td>19.84</td><td>5.3%</td></tr><tr><td>Sample 3</td><td>1372</td><td>1213</td><td>1353</td><td>1369</td><td>1326</td><td>66.06</td><td>4.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Low affinity immunoglobulin gamma Fc region receptor II-c;IgG Fc receptor II-c;CDw32;Fc-gamma RII-c;Fc-gamma-RIIc;FcRII-c;CD32;FCGR2C;CD32, FCG2, IGFR2;FCGR2B

Gene Name

Fc fragment of IgG receptor IIb/c

Gene ID

2213/9103

UniProt

P31994/P31995

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A46-P217

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

46.9 pg/ml - 3,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

35578 MW

Specificity

Natural and recombinant human FCGR2B/C

Anticoagulant

heparin or EDTA

Protein Name

Low affinity immunoglobulin gamma Fc region receptor II-b/c