Rat DKK-1 PicoKine® Quick ELISA Kit, Carrier-free

About this product

Rat DKK-1 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Rat Dkk1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK1432-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>91</td><td>93</td><td>93</td><td>92</td><td>92</td><td>0.82</td><td>0.8%</td></tr><tr><td>Sample 2</td><td>361</td><td>310</td><td>365</td><td>327</td><td>340</td><td>23.09</td><td>6.7%</td></tr><tr><td>Sample 3</td><td>1121</td><td>1338</td><td>1162</td><td>1204</td><td>1206</td><td>81.53</td><td>6.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

Dickkopf homolog 1 (Xenopus laevis) (Predicted) ;Dickkopf-like protein 1 ;Protein Dkk1 ;Dkk1 ;DKK1 , Dkk1_predicted , LOC100912050 ;rCG_48380 ;

Gene Name

dickkopf WNT signaling pathway inhibitor 1

Gene ID

293897

UniProt

D3Z9J1

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S23-H270

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

46.9 pg/ml - 3,000 pg/ml

Sensitivity

<15 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

29149 MW

Specificity

Natural and recombinant rat DKK1

Anticoagulant

heparin or EDTA

Protein Name

Dickkopf homolog 1