Rat IL-10 PicoKine® Quick ELISA Kit, Carrier-free

About this product

Rat IL-10/Interleukin-10 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Rat Il10 in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 4pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0418-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation. The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease. The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>55</td><td>58</td><td>57</td><td>49</td><td>54</td><td>3.49</td><td>6.4%</td></tr><tr><td>Sample 2</td><td>213</td><td>219</td><td>232</td><td>225</td><td>222</td><td>7.04</td><td>3.1%</td></tr><tr><td>Sample 3</td><td>839</td><td>901</td><td>925</td><td>826</td><td>872</td><td>41.39</td><td>4.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

Interleukin-10;IL-10;Cytokine synthesis inhibitory factor;CSIF;Il10;Il-10;

Gene Name

Interleukin-10

Gene ID

25325

UniProt

P29456

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S19-N178

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<4 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

20426 MW

Specificity

Natural and recombinant rat IL-10

Anticoagulant

EDTA

Protein Name

Interleukin-10