Mouse CXCL13 PicoKine® Quick ELISA Kit, Carrier-free

About this product

Mouse CXCL13 / BLC / BCA1 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Mouse Cxcl13 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0740-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

C-X-C motif chemokine 13 (CXCL13), also known as B lymphocyte chemoattractant (BLC), is a protein that in humans is encoded by theCXCL13 gene. CXCL13 is a small cytokine belonging to the CXC chemokine family, and it was located on sequence maps to 4q21, near the genes encoding most CXC chemokines. In T-lymphocytes, CXCL13 expression is thought to reflect a germinal center origin of the T-cell. Hence, expression of CXCL13 in T-cell lymphomas, such as Angioimmunoblastic T-cell Lymphoma, is thought to reflect a germinal center origin of the neoplastic T-cells.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>31</td><td>34</td><td>32</td><td>29</td><td>31</td><td>1.8</td><td>5.8%</td></tr><tr><td>Sample 2</td><td>182</td><td>204</td><td>199</td><td>194</td><td>194</td><td>8.16</td><td>4.2%</td></tr><tr><td>Sample 3</td><td>491</td><td>507</td><td>522</td><td>441</td><td>490</td><td>30.47</td><td>6.2%</td></tr></table>*number of samples for each test n=16.

Synonyms

C-X-C motif chemokine 13;B lymphocyte chemoattractant;CXC chemokine BLC;Small-inducible cytokine B13;Cxcl13;Blc, Scyb13;

Gene Name

C-X-C motif chemokine 13

Gene ID

55985

UniProt

O55038

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: I22-A109

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

11927 MW

Specificity

Natural and recombinant mouse CXCL13

Anticoagulant

heparin or EDTA

Protein Name

C-X-C motif chemokine 13