Mouse Cystatin C PicoKine® Quick ELISA Kit, Carrier-free

About this product

Mouse Cystatin C/CST3 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Mouse Cst3 in cell culture supernatants, serum, plasma ( heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0679-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Cystatin C or cystatin 3 (formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid (CSF) and of urine from patients with renal failure. Cystatin 3 has a low molecular weight (approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In mouses, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>437</td><td>434</td><td>446</td><td>503</td><td>455</td><td>28.06</td><td>6.1%</td></tr><tr><td>Sample 2</td><td>2310</td><td>2387</td><td>2135</td><td>2376</td><td>2302</td><td>100.81</td><td>4.3%</td></tr><tr><td>Sample 3</td><td>9815</td><td>8364</td><td>8977</td><td>9330</td><td>9121</td><td>528.93</td><td>5.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

Cystatin-C;Cystatin-3;Cst3;

Gene Name

Cystatin-C

Gene ID

13010

UniProt

P21460

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-A140

Applications

ELISA

Standard

20 ng/tube

Sample Type

cell culture supernatants, serum, plasma ( heparin, EDTA) and urine.

Detection Range

312 pg/ml - 20,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

15531 MW

Specificity

Natural and recombinant mouse Cystatin C

Anticoagulant

heparin or EDTA

Protein Name

Cystatin-C