Human CXCL16 PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human CXCL16 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human CXCL16 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0741-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Chemokine (C-X-C motif) ligand 16 (CXCL16) is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines (with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2. These are unusual features for a chemokine, and allow CXCL16 to be expressed as a cell surface bound molecule, as well as a soluble chemokine. CXCL16 is produced by dendritic cells found in the T cell zones of lymphoid organs, and by cells found in the red pulp of the spleen. Cells that bind and migrate in response to CXCL16 include several subsets of T cells, and natural killer T (NKT) cells. CXCL16 interacts with the chemokine receptorCXCR6, also known as Bonzo. The novel CXC-chemokine ligand 16 (CXCL16) functions as transmembrane adhesion molecule on the surface of APCs and as a soluble chemoattractant for activated T cells. Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha. The gene for human CXCL16 is located on chromosome 17.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>122</td><td>112</td><td>116</td><td>125</td><td>118</td><td>5.06</td><td>4.2%</td></tr><tr><td>Sample 2</td><td>725</td><td>748</td><td>679</td><td>765</td><td>729</td><td>32.29</td><td>4.4%</td></tr><tr><td>Sample 3</td><td>2351</td><td>2384</td><td>2122</td><td>2446</td><td>2325</td><td>122.47</td><td>5.2%</td></tr></table>*number of samples for each test n=16.

Synonyms

C-X-C motif chemokine 16;Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein;SR-PSOX;Small-inducible cytokine B16;Transmembrane chemokine CXCL16;CXCL16;SCYB16, SRPSOX;UNQ2759/PRO6714;

Gene Name

C-X-C motif chemokine 16

Gene ID

58191

UniProt

Q9H2A7

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: N49-P137

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

93.7 pg/ml - 6,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

27579 MW

Specificity

Natural and recombinant human CXCL16

Anticoagulant

heparin or EDTA

Protein Name

C-X-C motif chemokine 16