Human IL-2 PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human IL-2/Interleukin-2 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human IL2 in cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0397-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2 (IL2) is assigned to chromosome 4. Human IL-2 is a 15.5KDa glycoprotein, consisting of 153 amino acids in precursor form and 133 amino acids in mature form.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>31</td><td>29</td><td>28</td><td>30</td><td>29</td><td>1.11</td><td>3.8%</td></tr><tr><td>Sample 2</td><td>158</td><td>160</td><td>151</td><td>163</td><td>158</td><td>4.41</td><td>2.7%</td></tr><tr><td>Sample 3</td><td>484</td><td>469</td><td>488</td><td>480</td><td>480</td><td>7.08</td><td>1.4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Interleukin-2;IL-2;T-cell growth factor;TCGF;Aldesleukin;IL2;

Gene Name

Interleukin-2

Gene ID

3558

UniProt

P60568

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A21-T153

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<1 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

17628 MW

Specificity

Natural and recombinant human IL-2

Anticoagulant

heparin, EDTA or citrate

Protein Name

Interleukin-2