Human Cathepsin V PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human Cathepsin V PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human CTSV in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK1929-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Cathepsin L2, also known as cathepsin V and encoded by the CTSL2 gene, is a human gene. It is mapped to 9q22.33. The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may play an important role in corneal physiology. This gene is expressed in colorectal and breast carcinomas but not in normal colon, mammary gland, or peritumoral tissues, suggesting a possible role for this gene in tumor processes. Alternatively spliced variants, encoding the same protein, have been identified.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>100</td><td>121</td><td>111</td><td>116</td><td>112</td><td>7.77</td><td>6.9%</td></tr><tr><td>Sample 2</td><td>741</td><td>756</td><td>857</td><td>851</td><td>801</td><td>53.05</td><td>6.6%</td></tr><tr><td>Sample 3</td><td>2346</td><td>2384</td><td>2464</td><td>2369</td><td>2390</td><td>44.40</td><td>1.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Cathepsin L2; Cathepsin U; Cathepsin V; CTSV; CATL2; CTSL2; CTSU; UNQ268/PRO305

Gene Name

cathepsin V

Gene ID

1515

UniProt

O60911

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: V18-V334

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

78 pg/ml - 5,000 pg/ml

Sensitivity

<15 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant human Cathepsin V

Anticoagulant

heparin or EDTA