Human CCL16/HCC-4 PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human CCL16/HCC-4 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human CCL16 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK1127-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Chemokine (C-C motif) ligand 16 (CCL16) is a small cytokine belonging to the CC chemokine family that is known under several pseudonyms, including Liver-expressed chemokine (LEC) and Monotactin-1 (MTN-1). This chemokine is expressed by the liver, thymus, and spleen and is chemoattractive for monocytes and lymphocytes. Cellular expression of CCL16 can be strongly induced in monocytes by IL-10, IFN-gamma and bacterial lipopolysaccharide. Its gene is located on chromosome 17, in humans, among a cluster of other CC chemokines. CCL16 elicits its effects on cells by interacting with cell surface chemokine receptors such as CCR1, CCR2, CCR5 and CCR8.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>17</td><td>16</td><td>17</td><td>17</td><td>16</td><td>0.43</td><td>2.6%</td></tr><tr><td>Sample 2</td><td>60</td><td>53</td><td>52</td><td>58</td><td>55</td><td>3.34</td><td>6%</td></tr><tr><td>Sample 3</td><td>271</td><td>227</td><td>243</td><td>230</td><td>242</td><td>17.38</td><td>7.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

C-C motif chemokine 16;Chemokine CC-4;HCC-4;Chemokine LEC;IL-10-inducible chemokine;LCC-1;Liver-expressed chemokine;Lymphocyte and monocyte chemoattractant;LMC;Monotactin-1;MTN-1;NCC-4;Small-inducible cytokine A16;CCL16;ILINCK, NCC4, SCYA16;

Gene Name

C-C motif chemokine 16

Gene ID

6360

UniProt

O15467

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q24-Q120

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

7.8 pg/ml - 500 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

13600 MW

Specificity

Natural and recombinant human CCL16

Anticoagulant

heparin or EDTA

Protein Name

C-C motif chemokine 16