Human CCL25/TECK PicoKine® Quick ELISA Kit, Carrier-free

About this product

Human CCL25/TECK PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human CCL25 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK1145-carrier-free

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Chemokine (C-C motif) ligand 25 (CCL25) is a small cytokine belonging to the CC chemokine family that is also known as TECK (Thymus-Expressed Chemokine). The mouse Teck gene was mapped to chromosome 8 and human Teck gene was to 19p13.2. CCL25 is believed to play a role in the development of T-cells. It is chemotactic for thymocytes, macrophages, and dendritic cells. CCL25 elicits its effects by binding to the chemokine receptor CCR9

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>73</td><td>79</td><td>95</td><td>74</td><td>80</td><td>2.46</td><td>3.1%</td></tr><tr><td>Sample 2</td><td>482</td><td>414</td><td>421</td><td>310</td><td>407</td><td>15.25</td><td>3.8%</td></tr><tr><td>Sample 3</td><td>1099</td><td>1059</td><td>1181</td><td>1952</td><td>1323</td><td>78.7</td><td>6%</td></tr></table>*number of samples for each test n=16.

Synonyms

C-C motif chemokine 25;Chemokine TECK;Small-inducible cytokine A25;Thymus-expressed chemokine;CCL25;SCYA25, TECK;

Gene Name

C-C motif chemokine 25

Gene ID

6370

UniProt

O15444

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Applications

ELISA

Standard

10ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA).

Detection Range

62.5pg/ml-4000pg/ml

Sensitivity

<10pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

17 kDa

Specificity

Natural and recombinant human CCL25

Anticoagulant

heparin or EDTA

Protein Name

C-C motif chemokine 25