HBV-X Hepatitis B Virus X Recombinant Protein

About this product

HBV-X Recombinant produced in E. coli is a single polypeptide chain containing 165 amino acids (2-154) and having a molecular mass of 17.8 kDa. HBV-X is fused to a 12 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Specifications

Catalog number

PROTP12936-01

Size / count

5 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

Protein X

Gene Name

X

UniProt

P12936

Tag

His tag

Source

Escherichia coli

Sequence

[object Object]

Purification

Greater than 90%.

Reconstitution

Add 0.1M acetate buffer pH4 to prepare ~0.5mg/ml working stock solution. For higher pH, dilute with relevant buffer to 10µg/ml. The antigen has limited solubility in higher concentrations. Non-sterile product; filter before use in cell culture.

Function

Multifunctional protein that plays a role in silencing host antiviral defenses and promoting viral transcription. Does not seem to be essential for HBV infection. May be directly involved in development of cirrhosis and liver cancer (hepatocellular carcinoma) . Most of cytosolic activities involve modulation of cytosolic calcium. The effect on apoptosis is controversial depending on the cell types in which the studies have been conducted. May induce apoptosis by localizing in mitochondria and causing loss of mitochondrial membrane potential. May also modulate apoptosis by binding host CFLAR, a key regulator of the death-inducing signaling complex (DISC) . Promotes viral transcription by using the host E3 ubiquitin ligase DDB1 to target the SMC5-SMC6 complex to proteasomal degradation. This host complex would otherwise bind to viral episomal DNA, and prevents its transcription. Moderately stimulates transcription of many different viral and cellular transcription elements. Promoters and enhancers stimulated by HBx contain DNA binding sites for NF- kappa-B, AP-1, AP-2, c-EBP, ATF/CREB, or the calcium-activated factor NF-AT.

Shipping Conditions

Available

Storage Conditions

For long term storage, lyophilized protein should be stored at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Other Gene Names

Protein X

Subcellular Location

Host cytoplasm