Anti-MP2K1 Antibody

About this product

Boster Bio Anti-MP2K1 Antibody (Catalog # M00292-3) . Tested in WB, Flow Cytometry application (s) . This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00292-3-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator- activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.

Synonyms

Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, MKK1, 2.7.12.2, ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1, MAP2K1, MEK1, PRKMK1

Gene Name

MAP2K1

UniProt

Q02750

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

This MP2K1 antibody is generated from a mouse immunized with a recombinant protein between 1-393 amino acids from human MP2K1.

Clonality

Monoclonal

Clone

Clone: 1678CT373.38.18

Tissue Specificity

Widely expressed, with extremely low levels in brain.

Applications

WB, Flow Cytometry

Field of Research

Developmental Biology, Signal Transduction, TLR Signaling, T Cells, Chemokine signaling

Purification

This antibody is purified through a protein G column, followed by dialysis against PBS.

Function

29433126

Molecular Weight

43439 Da

Shipping Conditions

Available

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Fragment

IgG1, k

Subcellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cytoplasm. Nucleus Membrane; Peripheral membrane protein. Note=Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis (PubMed:14737111) . Membrane localization is probably regulated by its interaction with KSR1 (PubMed:10409742)