Anti-BBS5 Antibody (Center)

About this product

Boster Bio Anti-BBS5 Antibody (Center) (Catalog # A07197) . Tested in WB application (s) . This antibody reacts with Human.

Specifications

Catalog number

A07197-01

Size / count

80 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8 (GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for BBSome complex ciliary localization but not for the proper complex assembly.

Synonyms

Bardet-Biedl syndrome 5 protein, BBS5

Gene Name

BBS5

UniProt

Q8N3I7

Host

Rabbit

Reactivity

Human

Immunogen

This BBS5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 108-141 amino acids from the Central region of human BBS5.

Clonality

Polyclonal

Applications

WB

Field of Research

Neuroscience

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Dilution

WB: 1:1000

Function

The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8 (GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for BBSome complex ciliary localization but not for the proper complex assembly.

Molecular Weight

38755 Da

Shipping Conditions

Available

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Fragment

Rabbit IgG

Subcellular Location

Cell projection, cilium membrane. Cytoplasm. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite. Note=Localizes to basal bodies.

Prediction Reactivity

Monkey, Mouse