Recombinant Human FGF basic Protein (Trx Tag)

Specifications

Catalog number

PDEH100657-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury (TBI) . bFGF is a critical component of human embryonic stem cell culture medium. In addition, bFGF protein is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumour growth. Thus, bFGF is regarded as a target for cancers chemopreventive and therapeutic strategies.

Abbreviation

FGF basic

Synonyms

HBGF; FGF; BFGF; FGF-2; FGFB; HBGF-2; Basic FGF; FGF2; Basic Fibroblast Growth Factor; B-FGF; FGF-β; Fibroblast Growth Factor 2; heparin-binding growth factor 2; prostatropin; BFGF; B-FGF; FGF-2; FGFB; fibroblast growth factor 2; HBGF-2; heparin-binding growth factor 2; prostatropin; basic; Basic fibroblast growth factor bFGF; FGF 2; FGF B; FGF2 basic; Fibroblast growth factor; Fibroblast growth factor 2 (basic) ; HBGF 2; HBGF2; HBGH 2; HBGH2; Heparin binding growth factor 2 precursor; heparin-binding growth factor 2; FGF-β

UniProt

P09038

Accession Number

P09038

Expression System

E.coli

Tag

N-Trx

Sequence

Pro143-Ser288

Field of Research

Cardiovascular; Signal Transduction; Neuroscience; Stem Cells; Cancer; Developmental Biology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

36.1 kDa

Observed Molecular Weight

33 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human