FITC Conjugated Human CD5 Recombinant Rabbit Monoclonal Antibody [PSH05-01]

About this product

CD5 is a cluster of differentiation expressed on the surface of T cells (various species) and in a subset of murine B cells known as B-1a. The expression of this receptor in human B cells has been a controversial topic and to date there is no consensus regarding the role of this receptor as a marker of human B cells. B-1 cells have limited diversity of their B-cell receptor due to their lack of the enzyme terminal deoxynucleotidyl transferase (TdT) and are potentially self-reactive. CD5 serves to mitigate activating signals from the BCR so that the B-1 cells can only be activated by very strong stimuli (such as bacterial proteins) and not by normal tissue proteins. CD5 was used as a T-cell marker until monoclonal antibodies against CD3 were developed. In humans, the gene is located on the long arm of chromosome 11. There is no confirmed ligand for CD5 but there is evidence that CD72, a C-type lectin, may be a ligand or that CD5 may be homophilic, binding CD5 on the surface of other cells. CD5 includes a scavenger receptor cysteine-rich protein domain. T cells express higher levels of CD5 than B cells. CD5 is upregulated on T cells upon strong activation. In the thymus, there is a correlation with CD5 expression and strength of the interaction of the T cell towards self-peptides.

Specifications

Catalog number

HA720211F

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

CD 5 antibody CD5 antibody CD5 antigen (p56 62) antibody CD5 antigen antibody CD5 molecule antibody CD5_HUMAN antibody LEU1 antibody LyA antibody Lymphocyte antigen T1/Leu-1 antibody OTTHUMP00000236973 antibody T-cell surface glycoprotein CD5 antibody T1 antibody

Abbreviation

CD5, LEU1

Swiss Prot

P06127 Human

Cellular Locus

Cell membrane.

Host

Rabbit

Species Reactivity

Human

Immunogen

Recombinant protein within human CD5 aa 25-372 / 495.

Isotype

IgG

Clone

PSH05-01

Conjugation

FITC-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

FC

Positive Control

Human peripheral blood.

Dilution

FC: 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

Supplied in phosphate-buffered solution, pH 7.2, containing 0.2% ProClean 950 and BSA.

Molecular Weight

Predicted band size: 55 kDa

Storage Conditions

Store at 2°C to 8°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes