Alkaline Phosphatase Conjugate Stabilizer, 1X

Catalog number 6273

Discontinued

Price for 6273

Size / count 1 L

This variant is discontinued and cannot be added to your cart.

About this product

Alkaline Phosphatase Conjugate Stabilizer is a proprietary formulation to stabilize and dilute alkaline phosphatase conjugates for use in ELISA, Western blot, and other immunoassay applications. This formulation minimizes non-specific interactions and reduces background.

Specifications

Catalog number: 6273
Size / count: 1 L
Dry ice: No
Hazardous: No
Discontinued: Yes
Label: ICT
Type: ELISA & Assay Reagents
Assay Protocol: 1. Allow product to equilibrate to room temperature (25°C) prior to use., 2. Add 100 µL to each Well. When reacting with peroxidase, a soluble blue reaction product develops that can be read at 370 nm or in a range of 620-650 nm. The reaction product is stable for 1 hour., 3. The reaction can be stopped using an appropriate stop solution, such as Stop Solution for TMB Substrates (STOPT), for endpoint assays. STOPT changes the chromogen from blue to yellow and the absorbance to 450 nm. For best results, sample absorbance should be monitored and read before values exceed 2.0 OD units. STOPT can increase the absorbance up to 3-fold, so the reaction should be stopped when absorbance values reach approximately 0.7 OD units.
Concentration: 1X
pH: pH: 5.5-6.5
Additionnal Information: ELISA & Assay Reagents ; Conjugates and Stabilizers
Shipping Conditions: Ships overnight (domestic), International Priority Shipping
Storage Temperature: 2-8°C
Notes: 20% discount
Shelf Life: Expires two years from date of manufacture
Target Description: Alkaline Phosphatase Conjugate Stabilizer is a proprietary formulation to stabilize and dilute alkaline phosphatase conjugates for use in ELISA, Western blot, and other immunoassay applications., , This AP stabilizer can serve as a diluent for antigens and antibodies conjugated to alkaline phosphatase. This buffered formulation aids to minimize non-specific interactions in immunoassays utilizing an alkaline phosphatase-conjugated antibody or antigen. Alkaline phosphatase conjugates are stable to a concentration of 2 µg per mL (low) in Alkaline Phosphatase Conjugate Stabilizer.

Frequently Asked Questions

What is the catalog number for Alkaline Phosphatase Conjugate Stabilizer, 1X?

The catalog number for Alkaline Phosphatase Conjugate Stabilizer, 1X is 6273.

Is Alkaline Phosphatase Conjugate Stabilizer, 1X in stock?

Alkaline Phosphatase Conjugate Stabilizer, 1X is currently made to order / not held in immediate stock. Contact us for lead time.

What is Alkaline Phosphatase Conjugate Stabilizer, 1X used for?

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.