BCIP/NBT 1-Component AP Membrane Substrate (SUBB)

About this product

BCIP/NBT 1-Component AP Membrane Substrate (SUBB) is a ready-to-use formulation containing BCIP(5-bromo-4-Chloro-3′-Indolyphosphate p-Toluidine Salt) and NBT (Nitro-Blue Tetrazolium Chloride) in a mildly alkaline buffer. SUBB is useful for immunoblotting applications where alkaline phosphatase-conjugated molecules are used for detection. NBT serves as the oxidant and BCIP is the AP substrate. SUBB reacts with AP yielding a dark blue reaction product.

Specifications

Catalog number

6281

Size / count

100 mL

Dry ice

No

Hazardous

No

Discontinued

Yes

Label

ICT

Type

ELISA & Assay Reagents

Assay Protocol

1. Allow product to equilibrate to room temperature (25°C) prior to use., 2. Add stop solution to plate Well. Equal volumes of TMB microWell substrate and stop solution should be used. We recommend using 100 µL of TMB substrate and 100 µL of Stop Solution for TMB Substrates per Well. The stopped TMB reaction product is stable for 1 hour., 3. Read absorbance at 450 nm within 60 minutes. For best results, sample absorbance should be monitored and stopped before values exceed 2.0 OD units.

Concentration

1X

pH

pH: 9.6-10

Additionnal Information

ELISA & Assay Reagents ; Substrates

Shipping Conditions

Ships overnight (domestic), International Priority Shipping

Storage Temperature

2-8°C

Notes

20% discount

Shelf Life

3 years from date of manufacture

Target Description

BCIP/NBT 1-Component AP Membrane Substrate (SUBB) is a ready-to-use formulation containing BCIP(5-bromo-4-Chloro-3′-Indolyphosphate p-Toluidine Salt) and NBT (Nitro-Blue Tetrazolium Chloride) in a mildly alkaline buffer., , SUBB is useful for immunoblotting applications where alkaline phosphatase-conjugated molecules are used for detection. NBT serves as the oxidant and BCIP is the AP substrate. SUBB reacts with AP yielding a dark blue reaction product., , Bulk volumes are available upon request.