Stop Solution for TMB Substrates (STOPT)

Catalog number 6282

Discontinued

Price for 6282

Size / count 100 mL

This variant is discontinued and cannot be added to your cart.

About this product

Stop Solution for TMB Substrates (STOPT) is a ready-to-use liquid acidic stop solution suitable for use with our TMB substrates. TMB substrates react with peroxidase, yielding a soluble blue reaction product. When the TMB-peroxidase reaction is stopped using Stop Solution for TMB Substrates, the chromogen changes from blue to yellow. This product is suitable for all endpoint ELISAs using a TMB substrate for color development.

Specifications

Catalog number: 6282
Size / count: 100 mL
Dry ice: No
Hazardous: No
Discontinued: Yes
Label: ICT
Type: ELISA & Assay Reagents
Assay Protocol: 1. Reconstitute STOPP by adding 100 mL DiH2O and mix Well., 2. Add stop solution to plate Well. Equal volumes of stop solution and AP microWell substrate should be used. We recommend using 100 µL of pNPP substrate (SUBP) and 100 µL of STOPP per Well. The stopped reaction is stable for 1 hour., 3. Read absorbance at 405-420 nm within 60 minutes. For best results, sample absorbance should be monitored and stopped before values exceed 2.5 OD units.
Concentration: 1X
pH: pH: ≤ 2
Additionnal Information: ELISA & Assay Reagents ; STOP Solutions
Shipping Conditions: Ships via FedEx Ground (domestic only); this product cannot be shipped internationally. Please note: If other items are purchased with this product, there will be separate shipping fees for different shipping methods.
Storage Temperature: Room temperature
Notes: Domestic Shipping Only; 20% discount
Target Description: Stop Solution for TMB Substrates (STOPT) is a ready-to-use liquid acidic stop solution suitable for use with our TMB substrates. This product is suitable for all endpoint ELISAs using a TMB substrate for color development., , TMB substrates react with peroxidase, yielding a soluble blue reaction product. When the TMB-peroxidase reaction is stopped using Stop Solution for TMB Substrates, the chromogen changes from blue to yellow, and hence the absorbance from 620 nm to 450 nm., , Bulk volumes are available upon request.

Frequently Asked Questions

What is the catalog number for Stop Solution for TMB Substrates (STOPT)?

The catalog number for Stop Solution for TMB Substrates (STOPT) is 6282.

Is Stop Solution for TMB Substrates (STOPT) in stock?

Stop Solution for TMB Substrates (STOPT) is currently made to order / not held in immediate stock. Contact us for lead time.

What is Stop Solution for TMB Substrates (STOPT) used for?

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.

Does this product need any special customs documentation to be imported to my country?

If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.

What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.