Recombinant Mouse CCL3

About this product

Chemokine ligand 3 (CCL3), also known as Macrophage inflammatory protein-1 alpha (MIP-1 alpha) is a small cytokine belonging to the CC chemokine family. CCL3/MIP-1 alpha is a chemoattractant for several different leukocytes, with varying degrees of potency (monocytes = T-cells>neutrophils>eosinophils).

Specifications

Catalog number

6655

Size / count

5 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Synonyms

Mouse ; CCL3

Label

ICT

Type

Recombinant Protein

Source

Yeast

Sequence

APFGADTPTA CCFSYVARQL SRKIVADYFE TSSQCSKPGV IFQTKKGRQV CANPTEDWVQ EYITDLELNA (70)

Assay Protocol

1. Reconstitute each vial of Nigericin with 100 µL DMSO to form the 5 mM stock concentrate. Once reconstituted, it may be aliquoted and stored at ≤ -20°C for 1 year protected from light and thawed no more than twice during that time., 2. Immediately prior to addition to the samples and controls, dilute 5 mM Nigericin stock 1:10 in diH2O to form a 500 µM working solution for use in treating samples. For example, dilute 1:10 by adding 20 µL stock concentrate to 180 µL diH2O., 3. Use Nigericin at 1-20 µM to induce NLRP3 inflammasome activation in cells. For example, to use at 10 µM, dilute 500 µM working solution 1:50 in samples; e.g., spike 294 µL cell suspension/overlay medium with 6 µL of 500 µM working solution. Typical treatment periods range from 3-24 hours at 37°C. Each investigator should adjust the concentration of Nigericin and treatment period to accommodate the particular cell line and research conditions., 4. NLRP3 inflammasome activation can be detected by ELISA or Western blot measuring secreted pro-inflammatory cytokines IL-1β or IL-18, or through the use of caspase-1 activation assays, such as ICT’s Caspase-1 Assay Kits or Pyroptosis/Caspase-1 Assay Kit.

Form

Lyophilized

Shipping Conditions

Shipping: Ships at ambient temperature, Ships overnight (domestic), International Priority Shipping

Storage Temperature

-20°C

Notes

20% discount

Calculated Molecular Weight

7.9 kDa

Target Description

Chemokine ligand 3 (CCL3), also known as Macrophage inflammatory protein-1 alpha (MIP-1 alpha) is a small cytokine belonging to the CC chemokine family. There are at least 27 distinct members of the C-C subgroup reported for mammals. They are characterized by two adjacent cysteines. CC chemokines induce the migration of monocytes and other cell types such as NK cells and dendritic cells. CCL3/MIP-1 alpha is a chemoattractant for several different leukocytes, with varying degrees of potency (monocytes = T-cells>neutrophils>eosinophils).