Protein-Free Sample Diluent

About this product

Protein-Free Sample Diluent is a protein-free matrix used for the dilution of biological samples (e.g. serum, cell culture media) into the useful range of antibody-sandwich or antigen-down ELISA format assays. This unique buffer contains a heterogeneous mixture of proprietary molecules that help reduce background noise associated with non-specific bridging of signal-generating conjugates to the plate Well surface.

Specifications

Catalog number

6704

Size / count

1 L

Dry ice

No

Hazardous

No

Discontinued

Yes

Label

ICT

Type

ELISA & Assay Reagents

Assay Protocol

1. Protect the HRP Conjugate Stabilizer from light. This may be done by covering the container with aluminum foil., 2. Add the HRP-conjugated protein at the target concentration to HRP Conjugate Stabilizer. For example, if diluting an HRP-conjugated antibody 1:5000, add 10 µL conjugate stock to 49.99 mL HRP Conjugate Stabilizer., 3. Mix for 15 minutes., 4. Use the diluted HRP conjugate in the immunoassay., 5. Store remaining HRP conjugate solution at 2-8°C. Protect from direct exposure to light.

Concentration

1X ready to use

pH

pH 7.1-7.6

Additionnal Information

ELISA & Assay Reagents ; Sample and Assay Diluents

Shipping Conditions

Ships overnight (domestic), International Priority Shipping

Storage Temperature

2-8°C

Shelf Life

Expires two years from date of manufacture

Target Description

Sample diluents are used to dilute samples into the functional range of the assay and to create the standard curve. Due to the finite binding capacity of the plate Well-coated proteins (e.g., antibodies, antigens), highly concentrated samples must be diluted in order to obtain absorbance readings within the sensitivity detection limits of the instrument. Properly formulated sample diluents will also reduce background noise associated with non-specific bridging of signal-generating conjugates to the plate Well surface., , Protein-Free Sample Diluent provides a non-protein-based buffered, neutral pH environment that is highly compatible with antibody-antigen interactions. Antimicrobial agents allow for room temperature bench-top use and extensive storage stability at 2-8°C.