Flow Cytometry Cytotoxicity Assay

Catalog number ACT100-2

Discontinued

Price for ACT100-2

Size / count 100 Tests

This variant is discontinued and cannot be added to your cart.

About this product

A non-radioactive cytotoxicity assay allowing phenotyping of a heterogeneous target cell population and detection of cytolytic activity at the cellular level, using flow cytometry.

Specifications

Catalog number: ACT100-2
Size / count: 100 Tests
Dry ice: No
Hazardous: No
Discontinued: Yes
Label: ICT
Cell Type: K562
Type: Cell Viability
Detection Method: Flow cytometry
Assay Protocol: https://cdn.shopify.com/s/files/1/0512/5793/4009/files/ACT1Protocol.pdf?v=1693429573
Components: Part # 4002: CFSE Membrane Stain, 4 Vials (2-8C), Part # 4003: 7AAD Live/Dead Stain, 3 Vials (2-8C), Part# 3003: 10X Dulbecco’s PBS w/Ca & Mg
Shipping Conditions: Ships overnight (domestic), International Priority Shipping
Storage Temperature: 2-8°C
Target Description: The immune system is capable of recognizing and destroying target cells, such as tumor or intracellular pathogen infected cells, through a process known as cell mediated cytotoxicity (CMC) or antibody-dependent cell-mediated cytotoxicity (ADCC). Evaluation of this CMC/ADCC activity is one of the most important immunoassays to monitor the status of the immune system. The most commonly used method to measure CMC/ADCC is a radioactive chromium-51 (51Cr) release assays. There are several disadvantages with this assay in that it is expensive, difficult to load certain cell types, strict environmental regulations makes waste disposable expensive and spontaneous release of 51Cr results in high background. With the use of flow cytometry, it is now possible to eliminate the need for radioactive material and increased the ability to quantify cytolytic activity on a single cell bases. Various groups have demonstrated that measuring CMC/ADCC activity by flow cytometry has a strong (95%) correlation with the traditional 51Cr release assay. A cell tracking dye CFSE analog is utilized to label the target cell population and thus separating them form the effecter cell population. After the assay has run its experimental protocol, 7AAD (live/dead) is added to measure cell death. 7AAD only enters membrane compromised cells and binds to DNA. Flow cytometry is utilized to gate on the target cells and measure 7AAD negative vs. 7AAD positive cells.

Frequently Asked Questions

What is the catalog number for Flow Cytometry Cytotoxicity Assay?

The catalog number for Flow Cytometry Cytotoxicity Assay is ACT100-2.

Is Flow Cytometry Cytotoxicity Assay in stock?

Flow Cytometry Cytotoxicity Assay is currently made to order / not held in immediate stock. Contact us for lead time.

What is Flow Cytometry Cytotoxicity Assay used for?

A non-radioactive cytotoxicity assay allowing phenotyping of a heterogeneous target cell population and detection of cytolytic activity at the cellular level, using flow cytometry.

How many freeze-thaw cycles can this product tolerate?

We recommend aliquoting the product upon receipt to avoid repeated freeze-thaw cycles, which can reduce activity and stability. This is your responsibility once the product has been delivered.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

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