Bioluminescent Acetylcholinesterase Assay Kit

About this product

A rapid and extremely sensitive assay for acetylcholinesterase using the natural substrate acetylcholine. A series of coupled reactions generate a bioluminescent signal. Ideal for identifying and characterizing acetylcholinesterase inhibitors.

Specifications

Catalog number

CLACHE100-3

Size / count

500 Tests

Dry ice

No

Hazardous

No

Discontinued

Yes

Label

ICT

Type

Cell Viability

Detection Method

Luminometer

Assay Protocol

https://cdn.shopify.com/s/files/1/0512/5793/4009/files/aCella-AChEProtocol.pdf?v=1685550101

Components

1. Component A: Contains Acetylcholinesterase Part# 3023 x 5, 2. Component B: Contains Detection reagent, acetylcholine and coupled enzyme reaction Part# 3024 x 5, 3. Component C: Control to measure maximum Luminescence Part# 3025 x 5

Shipping Conditions

Ships overnight (domestic), International Priority Shipping

Storage Temperature

-20°C

Target Description

Acetylcholinesterase (AChE) is one of the most important enzymes involved in nerve transmission. The enzyme is bound to cellular membranes of excitable tissue (synaptic junction, endoplasmic reticulum, etc.). Acute toxicity to humans and animals through inhibition of AChE by both nerve gases and an important class of pesticides has long been a field of intensive scientific investigation. AChE inhibitors have also been used clinically as Alzheimer’s treatments (e.g., tacrine (tetrahydroaminoacridine)) and are the subject of increasing interest in various disease processes and treatment strategies. However, both environmental detection of AChE inhibitors and development of modulators of AChE enzymatic activity as drugs have been hampered by the difficulty and complexity of the current assay methods. We have developed a highly sensitive, very rapid, extremely simple assay for AChE activity, using the natural substrate, acetylcholine. As shown in Figure 1, a series of coupled enzyme reactions quickly translates the presence of active AChE into a change in the luminance of the reaction. First (reaction I), acetylcholine is hydrolyzed by the AChE to yield acetate and choline. The acetate and choline then enter a coupled enzyme reaction (reaction II) that results in consumption of ATP, and finally the ATP concentration is measured by the Well-established luciferase method (reaction III). These reactions can occur simultaneously, and the result is generally obtained in five minutes or less. Inhibitors of AChE are readily detected by an increase in luminance due to reduced consumption of ATP.