aCella - Tox - an assay designed to measure cell cytoxicity

Catalog number CLATOX100-3L

Discontinued

Price for CLATOX100-3L

Size / count 500 Tests (with 5 Lumi Plates)

This variant is discontinued and cannot be added to your cart.

About this product

A bioluminescence, non-radioactive cytotoxicity assay designed to quantitatively measure the release of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from mammalian cell lines or bacterial cells.

Specifications

Catalog number: CLATOX100-3L
Size / count: 500 Tests (with 5 Lumi Plates)
Dry ice: No
Hazardous: No
Discontinued: Yes
Label: ICT
Cell Type: Ramos cells; Daudi Cells
Type: Cell Viability
Detection Method: Luminometer
Assay Protocol: https://cdn.shopify.com/s/files/1/0512/5793/4009/files/aCella-TOX_v1_3_Protocol.pdf?v=1679588911
Components: 1. 4X Enzyme Assay Reagent Part# 6001 x 1 (Storage: -20°C), 2. Enzyme Assay Diluent Part# 3008 x 1 (Storage: 2-8°C), 3. 50X Detection Reagent Part# 6002 x 1 (Storage: -20°C), 4. 5.5X Detection Assay Diluent Part# 3009 x 1 (Storage: -20°C), 5. Glyeraldehyde 3-Phosphate-G3P Part# 6003 x 1 (Storage: -20°C), 6. Lysis Buffer Part# 3035 x 1 (Storage: 2-8°C),
Shipping Conditions: Ships overnight (domestic), International Priority Shipping
Storage Temperature: Store contents as labeled
Target Description: GAPDH is an important enzyme in the glycolysis and gluconeogenesis pathways. This homotetrameric enzyme catalyzes the oxidative phosphorylation of D-glyceraldehyde-3-phosphate to 1,3-diphosphoglycerate in the presence of cofactor and inorganic phosphate. In the aCella-TOX reaction scheme the release of GAPDH is coupled to the activity of the enzyme 3-Phosphoglyceric Phosphokinase (PGK) to produce ATP. ATP is detected via the luciferase, luciferin Bioluminescence methodology. Further, aCella-TOX is a homogeneous cytotoxicity assay; alternatively in dual mode, aCella-TOX can measure cytotoxicity and cell viability in the same plate. Culture supernatants can also be removed from the original plate and assayed in a different plate, allowing kinetics runs to be set up. The assay is non-destructive, allowing the monitoring of additional parameters such as gene expression.

Frequently Asked Questions

What is the catalog number for aCella - Tox - an assay designed to measure cell cytoxicity?

The catalog number for aCella - Tox - an assay designed to measure cell cytoxicity is CLATOX100-3L.

Is aCella - Tox - an assay designed to measure cell cytoxicity in stock?

aCella - Tox - an assay designed to measure cell cytoxicity is currently made to order / not held in immediate stock. Contact us for lead time.

What is aCella - Tox - an assay designed to measure cell cytoxicity used for?

A bioluminescence, non-radioactive cytotoxicity assay designed to quantitatively measure the release of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from mammalian cell lines or bacterial cells.

What is the shelf life of this product?

Shelf life and expiration dates vary by product and are shown on the product listing and label. Products used beyond their expiration date are not eligible for performance-related returns or refunds, so please check the lot number and expiry before use.

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.