aCella - Tox - an assay designed to measure cell cytoxicity

Catalog number CLATOX100-4

Discontinued

Price for CLATOX100-4

Size / count 1000 Tests

This variant is discontinued and cannot be added to your cart.

About this product

A bioluminescence, non-radioactive cytotoxicity assay designed to quantitatively measure the release of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from mammalian cell lines or bacterial cells.

Specifications

Catalog number: CLATOX100-4
Size / count: 1000 Tests
Dry ice: No
Hazardous: No
Discontinued: Yes
Label: ICT
Cell Type: Ramos cells; Daudi Cells
Type: Cell Viability
Detection Method: Luminometer
Assay Protocol: https://cdn.shopify.com/s/files/1/0512/5793/4009/files/aCella-TOX_v1_3_Protocol.pdf?v=1679588911
Components: 1. 4X Enzyme Assay Reagent Part# 6001 x 1 (Storage: -20°C), 2. Enzyme Assay Diluent Part# 3008 x 1 (Storage: 2-8°C), 3. 50X Detection Reagent Part# 6002 x 1 (Storage: -20°C), 4. 5.5X Detection Assay Diluent Part# 3009 x 1 (Storage: -20°C), 5. Glyeraldehyde 3-Phosphate-G3P Part# 6003 x 1 (Storage: -20°C), 6. Lysis Buffer Part# 3035 x 1 (Storage: 2-8°C),
Shipping Conditions: Ships overnight (domestic), International Priority Shipping
Storage Temperature: Store contents as labeled
Target Description: GAPDH is an important enzyme in the glycolysis and gluconeogenesis pathways. This homotetrameric enzyme catalyzes the oxidative phosphorylation of D-glyceraldehyde-3-phosphate to 1,3-diphosphoglycerate in the presence of cofactor and inorganic phosphate. In the aCella-TOX reaction scheme the release of GAPDH is coupled to the activity of the enzyme 3-Phosphoglyceric Phosphokinase (PGK) to produce ATP. ATP is detected via the luciferase, luciferin Bioluminescence methodology. Further, aCella-TOX is a homogeneous cytotoxicity assay; alternatively in dual mode, aCella-TOX can measure cytotoxicity and cell viability in the same plate. Culture supernatants can also be removed from the original plate and assayed in a different plate, allowing kinetics runs to be set up. The assay is non-destructive, allowing the monitoring of additional parameters such as gene expression.

Frequently Asked Questions

What is the catalog number for aCella - Tox - an assay designed to measure cell cytoxicity?

The catalog number for aCella - Tox - an assay designed to measure cell cytoxicity is CLATOX100-4.

Is aCella - Tox - an assay designed to measure cell cytoxicity in stock?

aCella - Tox - an assay designed to measure cell cytoxicity is currently made to order / not held in immediate stock. Contact us for lead time.

What is aCella - Tox - an assay designed to measure cell cytoxicity used for?

A bioluminescence, non-radioactive cytotoxicity assay designed to quantitatively measure the release of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from mammalian cell lines or bacterial cells.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.