Poly Caspase FAM-VAD-FMK

About this product

This kit uses the green fluorescent probe FAM-VAD-FMK to irreversibly label active caspases within living cells. Caspase positive cells may be distinguished from caspase negative cells by flow cytometry or fluorescence microscopy.

Specifications

Catalog number

FAM100-1

Size / count

25 Tests

Dry ice

No

Hazardous

No

Discontinued

Yes

Target

Poly Caspase

Label

ICT

Type

Cell Viability

Detection Method

Flow Cytometry, Fluorescence Plate Reader, Fluorescence Microscopy

Assay Protocol

https://www.celltechnology.com/wp-content/uploads/2020/12/FAMFMKProtocol.pdf

Components

Lyophilized FAM Labeled Peptide (FAM-VAD-FMK), 1 vial (Part 8001), 10X Apoptosis Wash Buffer, 15 mL (Part # 3028), Fixative, 6 mL (Part # 3027), Propidium Iodide, 1 mL (Part# 4013)

Shipping Conditions

Ships overnight (domestic), International Priority Shipping

Storage Temperature

2-8°C

Target Description

Our FAM reagents use a novel approach to detect active caspases. The methodology is based on carboxyfluorescein labeled fluoromethyl ketone (FMK)-peptide inhibitors of caspases. These inhibitors are cell permeable and non-cytotoxic. Once inside the cell, the inhibitor binds covalently to the active caspase. Cells that contain bound inhibitor can be analyzed by flow cytometry or fluorescence microscopy. The FAM-VAD-FMK reagent provided in the kit irreversibly binds to activated caspases (caspase-1, -2, -3, - 4, -5, -6, -7, -8 and -9). Apoptosis is an evolutionarily conserved form of cell suicide, which follows a specialized cellular process. The central component of this process is a cascade of proteolytic enzymes called caspases. These enzymes participate in a series of reactions that are triggered in response to pro-apoptotic signals and result in cleavage of protein substrates, causing the disassembly of the cell. Caspases have been identified in organisms ranging from C. elegans to humans. The mammalian caspases play distinct roles in apoptosis and inflammation. In apoptosis, caspases are responsible for proteolytic cleavages that lead to cell disassembly (effector caspases), and are involved in upstream regulatory events (initiator caspases). An active caspase consists of two large (~20 kD) and two small (~10 kD) subunits to form two heterodimers which associate in a tetramer. As is common with other proteases, caspases are synthesized as precursors that undergo proteolytic maturation, either autocatalytically or in a cascade by enzymes with similar specificity. Caspase enzymes specifically recognize a 4 amino acid sequence (on their substrate) which necessarily includes an aspartic acid residue. This residue is the target for the cleavage reaction, which occurs at the carbonyl end of the aspartic acid residue. Caspases can be detected via immunoprecipitation, immuno-blotting techniques using caspase specific antibodies, or by employing fluorogenic substrates which become fluorescent upon cleavage by the caspase.