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Product details

Casp 3/7 FAM-DEVD-FMK 25 Tests

Catalog number FAM200-1

Discontinued

Price for FAM200-1

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Size / count 25 Tests

This variant is discontinued and cannot be added to your cart.

About this product

Non-cytotoxic assay arrests further apoptotic activity via caspase inhibition., Cell permeablity permits direct visualization of cytosolic apoptotic events., Apoptotic cell population does not diminish over time., Add reagent directly to cells. No special buffer or media needed. No preparation of cell lysates required. Simple wash procedure., Works in diverse cell lines: human, rodent, Drosophila., Can be performed in conjunction with Annexin staining, TUNEL, antibody staining, or with other APO LOGIX reagents on the same population of cells., Permits high through-put screening. Protocol can be adapted for ex vivo as Well as in situ experiments., Yields both quantitative and qualitative results. Gives strong signal with little background noise.

Specifications

Catalog number
FAM200-1
Size / count
25 Tests
Dry ice
No
Hazardous
No
Discontinued
Yes
Target
Caspase-3/7
Label
ICT
Cell Type
Jurkat
Type
Cell Viability
Detection Method
Flow Cytometry, Fluorescence Plate Reader, Fluorescence Microscopy
Assay Protocol
https://www.celltechnology.com/wp-content/uploads/2020/12/FAMFMKProtocol.pdf
Components
Lyophilized FAM Labeled Peptide (FAM-DEVD-FMK), 1 vial (Part 8002), 10X Apoptosis Wash Buffer, 15 mL (Part # 3028), Fixative, 6 mL (Part # 3027), Propidium Iodide, 1 mL (Part# 4013)
Shipping Conditions
Ships overnight (domestic), International Priority Shipping
Storage Temperature
2-8°C
Target Description
APO LOGIX Carboxyfluoroscein Caspase Detection Kits label active caspases in living cells undergoing apoptosis. Cell Technology’s probes utilize carboxyfluorescein(FAM)-labeled peptide fluoromethyl ketone (FMK) caspase inhibitors (FAM-peptide-FMK). These FAM-peptide-FMK compounds are both cell permeable and non-cytotoxic during the course of the assay and thus allow the detection of active caspases in living cell systems.

Frequently Asked Questions

What is the catalog number for Casp 3/7 FAM-DEVD-FMK 25 Tests?

The catalog number for Casp 3/7 FAM-DEVD-FMK 25 Tests is FAM200-1.

Is Casp 3/7 FAM-DEVD-FMK 25 Tests in stock?

Casp 3/7 FAM-DEVD-FMK 25 Tests is currently made to order / not held in immediate stock. Contact us for lead time.

What is Casp 3/7 FAM-DEVD-FMK 25 Tests used for?

Non-cytotoxic assay arrests further apoptotic activity via caspase inhibition., Cell permeablity permits direct visualization of cytosolic apoptotic events., Apoptotic cell population does not diminish over time., Add reagent directly to cells. No special buffer or media needed. No preparation of ce

Can this product be used for diagnostic or therapeutic purposes?

No. This product, like all items we supply, is intended for research laboratory use only and must not be used for diagnostics, therapeutics, treatment, or consumption by humans or animals.

What is the shelf life of this product?

Shelf life and expiration dates vary by product and are shown on the product listing and label. Products used beyond their expiration date are not eligible for performance-related returns or refunds, so please check the lot number and expiry before use.

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.

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