Fluorescent Total Cholesterol Detection Kit

Catalog number FLCHOL100-2

Discontinued

Price for FLCHOL100-2

Size / count 100 Tests

This variant is discontinued and cannot be added to your cart.

About this product

A simple one step assay which can be used either as a Fluorometric or Colorimetric assay to detect the level of total cholesterol in samples. Cholesterol esters are hydrolyzed by cholesterol esterase. Cholesterol is detected via cholesterol oxidase.

Specifications

Catalog number: FLCHOL100-2
Size / count: 100 Tests
Dry ice: No
Hazardous: No
Discontinued: Yes
Label: ICT
Type: Metabolic Assays
Detection Method: Absorbance or Fluorescence plate reader
Assay Protocol: https://cdn.shopify.com/s/files/1/0512/5793/4009/files/Total_Cholesterol_Protocol.pdf?v=1693431074
Components: 1. Cholesterol Probe (Part# 4022), Storage: -20°C, 2. Enzyme Mix (Part# 6023), Storage: -20°C, 3. 1x Reaction Buffer (Part# 3055), Storage 2-8°C, 4. Cholesterol Standard (Part# 7018), Storage: -20°C, 5. DMSO (Part# 7019), Storage 2-8°C
Shipping Conditions: Ships overnight (domestic), International Priority Shipping
Storage Temperature: Store contents as labeled
Target Description: Cholesterol is a lipid present in the cell membranes of eukaryotes and circulates in the blood stream. It is used in the biosynthesis of hormones, and plays an important role in cell signaling processes. Cholesterol exists as a free acid, as Well as, in the esterified form as cholesteryl esters. Elevated levels of cholesterol are indicated in atherosclerosis and heart disease, and are the subject of large amount of research focused on cholesterol metabolism. Quantitative determination of cholesterol in experimental samples is central to this research. This assay kit provides a simple, one-step fluorometric or colorimetric method for determination of total cholesterol in serum and plasma samples. The assay is based on an enzyme- coupled reaction that detects both free cholesterol and cholesterol esters. Cholesterol esters are hydrolyzed by cholesterol esterase into cholesterol, which is then oxidized by cholesterol oxidase to yield hydrogen peroxide and cholest-4-en-3-one (ketone). The hydrogen peroxide then reacts with the cholesterol probe (detection reagent) in a 1:1 stoichiometry to produce the stable fluorescent product.

Frequently Asked Questions

What is the catalog number for Fluorescent Total Cholesterol Detection Kit?

The catalog number for Fluorescent Total Cholesterol Detection Kit is FLCHOL100-2.

Is Fluorescent Total Cholesterol Detection Kit in stock?

Fluorescent Total Cholesterol Detection Kit is currently made to order / not held in immediate stock. Contact us for lead time.

What is Fluorescent Total Cholesterol Detection Kit used for?

Is this product suitable for live cell imaging?

Suitability for live cell imaging depends on the specific reagent and conjugate used. Check the datasheet for imaging-specific validation, or contact us for guidance.

Is this product covered by a money-back guarantee?

Yes, we guarantee a full refund or store credit if this product arrives damaged beyond usability, or has a genuine performance issue under proper usage and storage, provided you report it immediately with evidence. Claims after the expiration date or due to improper storage after receipt are excluded.

Can this product be used for diagnostic or therapeutic purposes?

No. This product, like all items we supply, is intended for research laboratory use only and must not be used for diagnostics, therapeutics, treatment, or consumption by humans or animals.