Eosinophil Peroxidase Detection Kit

About this product

This one step, no-wash assay utilizes a non-fluorescent detection reagent, which is oxidized in the presence of hydrogen peroxide and Eosinophil Peroxidase to produce its fluorescent analog. Read in absorbance or fluorescence formats. HTS adaptable.

Specifications

Catalog number

FLEPO100-2

Size / count

100 Tests

Dry ice

No

Hazardous

No

Discontinued

Yes

Label

ICT

Type

Metabolic Assays

Detection Method

Fluorescence plate reader

Assay Protocol

https://cdn.shopify.com/s/files/1/0512/5793/4009/files/Fluoro_EPOProtocol.pdf?v=1693430828

Components

Part# 4016: Detection Reagent, 1 Vial ('-20C), Part# 3002: 10X Assay Buffer, 60mL (2-8C), Part# 3012: Hydrogen Peroxide, 1000µL of a Stabilized Solution (2-8C), Part# 6016: Eosinophil Peroxidase, 1 Vial (100µL at 10Units/mL) (2-8C)

Shipping Conditions

Ships overnight (domestic), International Priority Shipping

Storage Temperature

Store contents as labeled

Target Description

Eosinophil peroxidase (EPO) is the most abundant enzyme found in eosinophils. It is the major cytotoxic agent released by activated eosinophils and uses hydrogen peroxide to generate reactive oxidants from halides and pseudo halide thiocyanate. Eosinophils peroxidase has been shown to have antimycobacterial activity, however it is also implicated in tissue damage that occurs in asthma and other diseases. Currently, the function of eosinophil involvement in the immune response is being redefined. Once considered a cell involved in host protection of parasitic infection, eosinophils multiple functions as leukocytes involved in the initiation and propagation of diverse inflammatory responses is being investigated. Eosinophils are further involved as modulators of innate and adaptive immunity. The Eosinophil Peroxidase Detection Kit utilizes a non-fluorescent detection reagent, which is oxidized in the presence of hydrogen peroxide and EPO to produce its fluorescent analog with excitation at 530-571 nm and emission at 590-600 nm. Note: We do not recommend using this kit for eosinophil tissue infiltration experiments unless eosinophils are specifically isolated from tissue digests. This assay is subject to interference by myeloperoxidase (neutrophil) contamination in lysates from tissue infiltration experiments.