Peroxynitrite Detection Kit

About this product

The kit uses a novel probe (NP3) with excellent sensitivity and specificity to detect peroxynitrite in live cells and animals. The probe readily passes the blood-brain-barrier for neurological assessment.

Specifications

Catalog number

FLHPF100-2

Size / count

150 Tests

Dry ice

No

Hazardous

No

Discontinued

Yes

Label

ICT

Type

Cell Viability

Detection Method

Fluorescence plate reader

Assay Protocol

https://cdn.shopify.com/s/files/1/0512/5793/4009/files/Fluoro_Peroxynitrite_Protocol.pdf?v=1693430932

Components

Part# 4012: HPF, 1 Vial (2-8C)

Shipping Conditions

Ships overnight (domestic), International Priority Shipping

Storage Temperature

2-8°C

Cellular Imaging & Detection

Oxidative Stress

Target Description

Peroxynitrite (ONOO- ) is produced via a diffusion reaction of nitric oxide and superoxide radicals. Endogenous Peroxynitrite is toxic to cells and affects mitochondrial function that can trigger cell death through nitration and oxidation reactions, however Peroxynitrite is also cytotoxic to invading pathogens. At the biochemical level, peroxynitrite is involved in protein, lipids and DNA modification via oxidation and nitration reactions. Protein modification can lead to chemical and physical changes of protein properties. Furthermore, DNA modification can lead to DNA strand breaks and cell death (apoptosis). Peroxynitrite modification of lipids can modulate lipid-protein adducts formation. Generation of peroxynitrite is attributed to pathogenic conditions in diseases such as neurodegenerative disorders, stroke, chronic inflammation, chronic heart failure, myocardial infarction and cancer. Redox related injuries are also related to peroxynitrite production like ischemia-reperfusion injury. Although peroxynitrite is a strong oxidant it reacts at a relative slow rate and can diffuse over a long range as far as on a cellular scale. These properties of peroxynitrite make it an interesting candidate for novel therapeutic interventions. We introduce NP3, a novel probe developed by Dr. Xin Li et.al at the Zhejian University, Hangzhou China. NP3 exhibits excellent sensitivity and specificity to peroxynitrite in live cells and animals and can readily pass the blood brain barrier.