Fluorescent Lactate Detection Kit

About this product

This kit provides a reliable, sensitive fluorometric method for the quantification of lactate in biological samples such as serum, plasma, urine, and tissue extracts. The assay plate can be incubated and read in 15-30 min.

Specifications

Catalog number

FLLACT100-2

Size / count

100 Tests

Dry ice

No

Hazardous

No

Discontinued

Yes

Label

ICT

Type

Metabolic Assays

Detection Method

Fluorescence plate reader

Assay Protocol

https://cdn.shopify.com/s/files/1/0512/5793/4009/files/Fluoro_Lactate_Protocol.pdf?v=1693430859

Components

Part # 7022: Lactate Standard 4mM, 500µl (2-8C), Part # 6004: Horseradish Peroxidase, 18.9 Units (2-8C), Part # 3011: 5X Reaction Buffer, 25 mL (2-8C), Part # 4026: Detection Reagent, 1 Vial (-20C), Part # 6025: Reaction Enzyme Mix, 1 Vial - 1.1mL (-20C)

Shipping Conditions

Ships overnight (domestic), International Priority Shipping

Storage Temperature

2-8°C

Target Description

Lactate is an intermediate product of carbohydrate metabolism. Of the two forms of Lactate, D- and L-, the L-lactate is the predominant isomer found in biological systems. L-lactate is formed during the anaerobic glycolysis by conversion of pyruvate to L-lactate by lactate dehydrogenase. Lactate level is an indicator for tissue oxygen demand and utilization. Abnormally high lactate levels are associated with diseases such as diabetes and lactate acidosis. Our lactate assay is a lactate oxidase-based method for detecting L-lactate in biological samples such as serum, plasma, blood, urine, and tissue extracts. In the assay, lactate oxidase (LOX) catalyzes the oxidation of L-lactate to pyruvate, along with the concomitant reduction of hydrogen peroxide (H2O2). The detection utilizes a non-fluorescent detection reagent, which is oxidized in the presence of horseradish peroxidase and LOX to produce its fluorescent analog.