Fluorescent Monoamine Oxidase Detection Kit

About this product

This kit measures monoamine oxidase activity using a non-fluorescent detection reagent to measure H2O2 produced from the conversion of a substrate to its aldehyde via MAO-A/B. The H2O2 oxidizes the reagent to the fluorescent product resorufin.

Specifications

Catalog number

FLMAO100-3

Size / count

500 Tests

Dry ice

No

Hazardous

No

Discontinued

Yes

Label

ICT

Type

Metabolic Assays

Detection Method

Fluorescence plate reader

Components

Part # 3020: 5X Reaction Buffer, 1 Bottle (2-8C), Part # 6007: Horseradish Peroxidase, 1 Vial (2-8C), Part # 7006: MAO-B Substrate, Benzylamine, 1 Vial (2-8C (Aliquot in Single Use Vials)), Part # 7005: MAO-A/B Substrate, Tyramine, 1 Vial (2-8C (Aliquot in Single Use Vials)), Part # 7002: Clorgyline: Monoamine Oxidase A Inhibitor, 1 Vial (2-8C (Aliquot in Single Use Vials)), Part # 7003: Pargyline: Monoamine Oxidase B Inhibitor, 1 Vial (-20C (Aliquot in Single Use Vials)), Part# 4009: Detection Reagent, 1 Vial (-20C (Aliquot in Single Use Vials))

Shipping Conditions

Ships overnight (domestic), International Priority Shipping

Storage Temperature

Store contents as labeled

Target Description

Monoamine oxidase (MAO) is a flavin-containing enzyme that catalyzes the oxidation of a variety of amine-containing neurotransmitters such as serotonin, norepinephrine, epinephrine and dopamine to yield the corresponding aldehydes. MAO exists in two isoforms, namely MAO-A and MAO-B, which are the products of two distinct genes. MAO-A and B exhibit different specificities to substrates and inhibitor selectivities. Extensive studies have been preformed to characterize their properties. MAO-A acts preferentially on serotonin and norepinephrine, and is inhibited by clorgyline. MAO-B acts preferentially on 2-phenylethylamine and benzylamine and is inhibited by deprenyl and pargyline. Localized in the outer mitochondrial membrane, these enzymes are found throughout the body. Often only one form of the enzyme is present in a specific organ and/or within a specific cell type. In addition to their role in regulating neurotransmitters, these enzymes are also involved in processing biogenic amines including tyramine. This kit utilizes a non-fluorescent detection reagent to measure H2O2 released from the conversion of a substrate to its aldehyde via MAO-A/B. Furthermore H2O2 oxidizes the detection reagent in a 1:1 stoichiometry to produce a fluorescent product resorufin. This oxidation is catalyzed by peroxidase. The fluorescent monoamine oxidase detection kit can be used to monitor MAO activity and the screening of MAO inhibitors.