Fluoro NAD/NADH Detection Kit by Cell Technology

Catalog number FLNADH100-2

Discontinued

Price for FLNADH100-2

Size / count 100 Tests

This variant is discontinued and cannot be added to your cart.

About this product

The kit uses a fluorescent readout to specifically detect NAD/NADH, with no reactivity with NADP/NADPH. It is suitable for use with cells and tissue from a range of sources such as animals, plants, bacteria and fungi.

Specifications

Catalog number: FLNADH100-2
Size / count: 100 Tests
Dry ice: No
Hazardous: No
Discontinued: Yes
Label: ICT
Type: Metabolic Assays
Detection Method: Fluorescence plate reader
Wavelength: 530-570nm/590-600nm
Assay Protocol: https://cdn.shopify.com/s/files/1/0512/5793/4009/files/Cell_Technology_Protocol_For_Fluoro_NAD.NADH.pdf?v=1693512674
Components: Part# 6021: Enzyme Mix x 1 (-20C), Part# 4018: NADH Detection Reagent x 1 (-20C), Part# 7013: NADH Standard x 1 (-20C), Part# 3044: NAD/NADH Reaction Buffer x 1 (2-8 C), Part# 3045: NAD/NADH Lysis Buffer x 1 (2-8 C), Part# 3046: NAD Extraction Buffer x 1 (2-8 C), Part# 3047: NADH Extraction Buffer x 1 (2-8 C), Part# 3051: NADH Standard Diluent x 1 (2-8 C)
Shipping Conditions: Ships overnight (domestic), International Priority Shipping
Storage Temperature: Store contents as labeled
Target Description: The role of pyridine nucleotides (NAD/NADH) metabolism in health is of continual and increased interest. A growing amount of evidence supports the fact that NAD metabolism regulates important biological effect including life span. NAD, through poly-ADP-ribosyl polymerase (PARP), mono-ADP-ribosyltransferase (ARTs) and recently characterized sirtuin enzymes, exerts potential biological effects. These enzymes modify proteins to regulate their function via ADP-ribosylation or deacetylation. These enzymes are involved in several pathways including apoptosis, DNA repair, senescence and endocrine signaling, which suggests that either the enzymes or NAD could be an important therapeutical target. The Fluoro NAD/NADH detection kit utilizes a non-fluorescent detection reagent, which is reduced in the presence NADH to produce its fluorescent analog and NAD. NAD is further converted to NADH via an enzyme-coupled reaction. The enzyme reaction specifically reacts with NAD/NADH and not with NADP/NADPH. The kit is suitable for applications such as: - Detection of NAD/NADH activity in cells or tissue extracts. - Detection of NAD/NADH levels in apoptosis, metabolism, proliferation, DNA repair, senescence, endocrine signaling and life span. - NAD/NADH detection in bacterial, fungal and plant cells.

Frequently Asked Questions

What is the catalog number for Fluoro NAD/NADH Detection Kit by Cell Technology?

The catalog number for Fluoro NAD/NADH Detection Kit by Cell Technology is FLNADH100-2.

Is Fluoro NAD/NADH Detection Kit by Cell Technology in stock?

Fluoro NAD/NADH Detection Kit by Cell Technology is currently made to order / not held in immediate stock. Contact us for lead time.

What is Fluoro NAD/NADH Detection Kit by Cell Technology used for?

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.

Can I get a trial-size or discounted first order of this product?

We don’t generally offer free samples, but trial discounts in exchange for feedback and validation results are almost always possible. Contact us with your intended application to arrange one.