Mito Flow - Mitochondrial membrane potential detection kit

Catalog number Flo200-2

Discontinued

Price for Flo200-2

Size / count 100 Tests

This variant is discontinued and cannot be added to your cart.

About this product

Flo200-2 is an easy to use kit which measures mitochondrial membrane potential using flow cytometry. Useful for analysing both suspension and monolayer adherent cell lines.

Specifications

Catalog number: Flo200-2
Size / count: 100 Tests
Dry ice: No
Hazardous: No
Discontinued: Yes
Label: ICT
Cell Type: Jurkat
Type: Cell Viability
Detection Method: Flow cytometry
Assay Protocol: https://cdn.shopify.com/s/files/1/0512/5793/4009/files/Mito_Flow_Protocol.pdf?v=1693431027
Components: Part # 4004: Vial of Mito Flow Dye x 1 (2 - 8 C), Part # 3004: 10X Dilution Buffer x 1 (2 - 8 C)
Shipping Conditions: Ships overnight (domestic), International Priority Shipping
Storage Temperature: 2-8°C
Cellular Imaging & Detection: Mitochondrial
Target Description: The loss of mitochondrial membrane potential is a hallmark for apoptosis. The mitochondrial permeability transition is an important step in the induction of cellular apoptosis. During this process, the electrochemical gradient across the mitochondrial membrane collapses. The collapse is thought to occur through the formation of pores in the mitochondria by dimerized Bax or activated Bid, Bak, or Bad proteins. Activation of these pro-apoptotic proteins is accompanied by the release of cytochrome c into the cytoplasm. The mitochondrial membrane potential detection kit assay utilizes cationic dye to visualize mitochondrial membrane potential. The reagent is a cell permeable cationic dye that has a strong fluorescent signal in the red region and exhibits low membrane potential independent (non specific) binding and toxicity. In healthy cells Mito Flow reagent is accumulated by the mitochondria in proportion to the DeltaPsi (membrane potential). In most cell lines, accumulation of the reagent in the mitochondria results in a higher fluorescence intensity. In apoptotic cells, where the mitochondrial membrane potential is compromised, the reagent does not accumulate in the mitochondria and these cells exhibit a lower fluorescence signal. Please see protocol for instructions and useful hints and tips.

Frequently Asked Questions

What is the catalog number for Mito Flow - Mitochondrial membrane potential detection kit?

The catalog number for Mito Flow - Mitochondrial membrane potential detection kit is Flo200-2.

Is Mito Flow - Mitochondrial membrane potential detection kit in stock?

Mito Flow - Mitochondrial membrane potential detection kit is currently made to order / not held in immediate stock. Contact us for lead time.

What is Mito Flow - Mitochondrial membrane potential detection kit used for?

Flo200-2 is an easy to use kit which measures mitochondrial membrane potential using flow cytometry. Useful for analysing both suspension and monolayer adherent cell lines.

What is the shelf life of this product?

Shelf life and expiration dates vary by product and are shown on the product listing and label. Products used beyond their expiration date are not eligible for performance-related returns or refunds, so please check the lot number and expiry before use.

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.