Mito Flow - Mitochondrial membrane potential detection kit

Catalog number Flo200-3

Discontinued

Price for Flo200-3

Size / count 500 Tests

This variant is discontinued and cannot be added to your cart.

About this product

Flo200-2 is an easy to use kit which measures mitochondrial membrane potential using flow cytometry. Useful for analysing both suspension and monolayer adherent cell lines.

Specifications

Catalog number: Flo200-3
Size / count: 500 Tests
Dry ice: No
Hazardous: No
Discontinued: Yes
Label: ICT
Cell Type: Jurkat
Type: Cell Viability
Detection Method: Flow cytometry
Assay Protocol: https://cdn.shopify.com/s/files/1/0512/5793/4009/files/Mito_Flow_Protocol.pdf?v=1693431027
Components: Part # 4004: Vial of Mito Flow Dye x 5 (2 - 8 C), Part # 3004: 10X Dilution Buffer x 5 (2 - 8 C)
Shipping Conditions: Ships overnight (domestic), International Priority Shipping
Storage Temperature: 2-8°C
Cellular Imaging & Detection: Mitochondrial
Target Description: The loss of mitochondrial membrane potential is a hallmark for apoptosis. The mitochondrial permeability transition is an important step in the induction of cellular apoptosis. During this process, the electrochemical gradient across the mitochondrial membrane collapses. The collapse is thought to occur through the formation of pores in the mitochondria by dimerized Bax or activated Bid, Bak, or Bad proteins. Activation of these pro-apoptotic proteins is accompanied by the release of cytochrome c into the cytoplasm. The mitochondrial membrane potential detection kit assay utilizes cationic dye to visualize mitochondrial membrane potential. The reagent is a cell permeable cationic dye that has a strong fluorescent signal in the red region and exhibits low membrane potential independent (non specific) binding and toxicity. In healthy cells Mito Flow reagent is accumulated by the mitochondria in proportion to the DeltaPsi (membrane potential). In most cell lines, accumulation of the reagent in the mitochondria results in a higher fluorescence intensity. In apoptotic cells, where the mitochondrial membrane potential is compromised, the reagent does not accumulate in the mitochondria and these cells exhibit a lower fluorescence signal. Please see protocol for instructions and useful hints and tips.

Frequently Asked Questions

What is the catalog number for Mito Flow - Mitochondrial membrane potential detection kit?

The catalog number for Mito Flow - Mitochondrial membrane potential detection kit is Flo200-3.

Is Mito Flow - Mitochondrial membrane potential detection kit in stock?

Mito Flow - Mitochondrial membrane potential detection kit is currently made to order / not held in immediate stock. Contact us for lead time.

What is Mito Flow - Mitochondrial membrane potential detection kit used for?

Flo200-2 is an easy to use kit which measures mitochondrial membrane potential using flow cytometry. Useful for analysing both suspension and monolayer adherent cell lines.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.