Fluoro Catalase Activity Detection Kit by Cell Technology

Catalog number FLOCAT100-3

Discontinued

Price for FLOCAT100-3

Size / count 500 Tests

This variant is discontinued and cannot be added to your cart.

About this product

A sensitive, one-step, no wash assay for the sensitive monitoring of catalase activity by measuring residual hydrogen peroxide substrate. A non-fluorescent detection reagent generates a fluorescent signal in the presence of peroxidase and resorufin.

Specifications

Catalog number: FLOCAT100-3
Size / count: 500 Tests
Dry ice: No
Hazardous: No
Discontinued: Yes
Label: ICT
Type: Metabolic Assays
Detection Method: Fluorescence plate reader
Assay Protocol: https://cdn.shopify.com/s/files/1/0512/5793/4009/files/Cell_Technology_protocol_for_Fluoro_Catalase_FLOCAT100.pdf?v=1693512646
Components: 3021: 5X Reaction Buffer pH 7.4, 25mL (2-8C), 4010: Detection Reagent, 1 Vial (2-8C - Undiluted; Diluted: -20C(Aliquot in Single Use Vials)), 6009: Horseradish Peroxidase, 1 Vial (2-8C - Undiluted; Diluted: -20C), 3022: Hydrogen Peroxide, 1 Vial (2-8C), 6008: Catalase Enzyme, 1 Vial (2-8C (Crystalline Suspension))
Shipping Conditions: Ships overnight (domestic), International Priority Shipping
Storage Temperature: 2-8°C
Target Description: Catalase is an antioxidant enzyme that catalyzes the decomposition of hydrogen peroxide (H2O2) to water and oxygen. Catalase is ubiquitously expressed in mammalian and non‐mammalian aerobic cells containing the cytochrome system. The enzyme has been isolated from various sources, including bacteria and plant cells. Catalase activity varies greatly from tissue to tissue. Highest activity is seen in liver and kidney, while lowest activity is seen in connective tissue. In eukaryotic cells, catalase in concentrated in organelles called peroxisomes. The production of hydrogen peroxide in eukaryotic cells is an end product result of various oxidases and superoxide dismutase reactions. Accumulation of H2O2 can result in cellular damage through oxidation of proteins, DNA and lipids thus resulting in cell death and mutagenesis. Hydrogen peroxide’s role in oxidative stress related diseases has been widely studied. The Fluoro Catalase detection kit is a sensitive assay that utilizes a non‐fluorescent detection reagent to measure H2O2 substrate left over from the catalase reaction.

Frequently Asked Questions

What is the catalog number for Fluoro Catalase Activity Detection Kit by Cell Technology?

The catalog number for Fluoro Catalase Activity Detection Kit by Cell Technology is FLOCAT100-3.

Is Fluoro Catalase Activity Detection Kit by Cell Technology in stock?

Fluoro Catalase Activity Detection Kit by Cell Technology is currently made to order / not held in immediate stock. Contact us for lead time.

What is Fluoro Catalase Activity Detection Kit by Cell Technology used for?

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.