Hydrogen Peroxide Detection/Peroxidase Detection Kit

About this product

A rapid, no-wash assay to measure H2O2, based on the formation of a fluorescent product. The kit can measure H2O2 release from cells or enzyme coupled reactions. It can also measure peroxidase activity.

Specifications

Catalog number

FLOH100-3

Size / count

500 Tests

Dry ice

No

Hazardous

No

Discontinued

Yes

Label

ICT

Type

Cell Viability

Detection Method

Fluorescence plate reader

Assay Protocol

https://cdn.shopify.com/s/files/1/0512/5793/4009/files/Cell_Technology_Protocol_for_Fluoro_H2O2_FLOH100-3_Detection_Kit.pdf?v=1693512640

Components

Part # 3011: 5X Reaction Buffer: 20 ml buffer, pH 7.4 (2-8°C), Part # 4007: Detection reagent: One vial for 500 assays. (2-8°C), Part # 3012: Hydrogen Peroxide: 200µL of a stabilized 3% solution. (2-8°C), Part # 6004: Horseradish Peroxidase: 18.9 Units of enzyme (2-8°C)

Shipping Conditions

Ships overnight (domestic), International Priority Shipping

Storage Temperature

2-8°C

Cellular Imaging & Detection

Oxidative Stress

Target Description

The kit utilizes a non-fluorescent detection reagent to measure H2O2. H2O2 oxidizes the detection reagent to produce a fluorescent product, resorufin which is catalyzed by peroxidase in a homogeneous no wash assay system. The detection reagent can be utilized to measure H2O2 release from cells or enzyme coupled reactions.