Fluorescent Phosphate Detection Kit

About this product

This kit provides a simple, one-step fluorometric or colorimetric method for determination of phosphate in serum and plasma samples. The assay is based on an enzyme-coupled reaction that detects inorganic phosphate.

Specifications

Catalog number

FLPHOS100-2

Size / count

100 Tests

Dry ice

No

Hazardous

No

Discontinued

Yes

Label

ICT

Type

Metabolic Assays

Detection Method

Fluorescence plate reader or spectrophotometer

Wavelength

535/585nm or Absorbance readout @ 570nm

Assay Protocol

https://cdn.shopify.com/s/files/1/0512/5793/4009/files/Fluoro_Phosphate_Protocol.pdf?v=1693430912

Components

Part # 7023: 25X Substrate (-20C), Part # 4025: Detection Reagent (-20C), Part # 6026: Enzyme A (-20C), Part # 6027: Enzyme B (-20C), Part # 3060: Phosphate Standard (2-8C), Part # 6028: Horseradish Peroxidase Enzyme (2-8C), Part # 3059: 1X Reaction Buffer (2-8C), Part # 3061: Sample Diluent (2-8C), 1 Black Clear-Bottom 96-Well Plate for Fluorescent Plate Reader

Shipping Conditions

Ships overnight (domestic), International Priority Shipping

Storage Temperature

Store contents as labeled

Target Description

Phosphorus is essential for multiple and diverse biological functions, including cellular signal transduction, mineral metabolism, and energy exchange. Although >80% of total body phosphorus is stored in bone and teeth, intracellular phosphorus exists in the form of organic compounds such as ATP and as free anions like H3PO43-, which are commonly referred to as phosphate. Serum phosphorus primarily occurs in the form of inorganic phosphate, which is maintained within the physiological range by regulation of dietary absorption, bone formation, and renal excretion, as Well as equilibrium with intracellular stores. Elevated levels of serum phosphate may be associated with a higher risk of death and adverse cardiovascular outcomes in people with prior myocardial infarction, and is the subject of large amount of research. Rapid and easy quantification of serum phosphate in experimental samples may prove clinically useful. The Fluorescent Phosphate Detection Kit provides a simple, one-step fluorometric or colorimetric method for determination of phosphate in serum and plasma samples. The assay is based on an enzyme-coupled reaction that detects inorganic phosphate. The substrate is converted to Hydrogen peroxide in presence of inorganic phosphate in a coupled enzymatic reaction. The Hydrogen Peroxide then reacts with the detection reagent in a 1:1 stoichiometry in presence of Horse Radish Peroxidase to produce the stable fluorescent product.